Biochemical Properties of Trypanosomatid Lactate Dehydrogenases
نویسندگان
چکیده
منابع مشابه
Biochemical properties of trypanosomatid lactate dehydrogenases.
Lactate dehydrogenase (LDH, E.C.1.1.1.27) was found in supernatant (cytoplasmic enzyme) fractions of the trypanosomatid flagellates Trypanosoma conorhini and Crithidia fasciculata if 10 mm cysteine was present in the homogenizing medium. The T. conorhini LDH activity with pyruvate as substrate was increased 35% if 5 mm cysteine was also included in reaction mixtures. K(m) values for the T. cono...
متن کاملPhysical and chemical properties of reversibly inactivated lactate dehydrogenases.
The effect of reversible inactivation on the physical and chemical properties of dogfish M4 and chicken heart H* lactate dehydrogenases has been studied. Reduced diphosphopyridine nucleotide was found to have a significant effect on the rate of recovery of the enzymatic activity, but not on the over-all recovery. Optical rotatory dispersion, circular dichroism, fluorescence, and immunological s...
متن کاملMembrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Purification and properties.
Procedures were developed for the optimal solubilization of D-lactate dehydrogenase, D-mandelate dehydrogenase, L-lactate dehydrogenase and L-mandelate dehydrogenase from wall + membrane fractions of Acinetobacter calcoaceticus. D-Lactate dehydrogenase and D-mandelate dehydrogenase were co-eluted on gel filtration, as were L-lactate dehydrogenase and L-mandelate dehydrogenase. All four enzymes ...
متن کاملThe lactate dehydrogenases of hemopoietic cells.
By W. H. STARKWEATHER, H. H. SPENCER AND H. K. ScriocH M AMMALIAN LACTATE DEHYDROGENASE ( LDH ) can be separated into five active fractions.’4 The production of electrophoretic extremes, LDH-1 (H4 ) and LDH-5 ( M4 ), is controlled by the action of two genes.5’#{176}The intermediate fractions ( LDH-2, LDH-3, LDH-4 ) are considered hybrid forms of LDH-1 and LDH-5.5’” Assuming that the hybrids are...
متن کاملSubunit hybridization of higher plant lactate dehydrogenases
developed a procedure, based on the affinity of all three enzymes for Cibacron Blue 3GA, which allows their simultaneous purification from a cell extract. E. coli K 12, EMG-2 was grown, harvested and disrupted as described by Miller & Stadtman (1972). After treatment with 1% (w/v) streptomycin sulphate, the cell extract was subjected to ammonium sulphate fractionation. The protein fraction prec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1970
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.102.3.826-834.1970